化学生物総合管理

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第6巻 , 第1号 , (pp.7-14)
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細菌の生物活性を利用したカキからのノロウイルス検査法の改良
秋場哲哉、尾畑浩魅、林志直、森功次、野口やよい、永野美由紀、仲真晶子、甲斐明美、矢野一好
東京都健康安全研究センター
Improvement of Norovirus Detection Method using Bacteria from Oysters

Tetsuya AKIBA Hiromi OBATA Yukinao HAYASHI Kohji MORI Yayoi NOGUCHI Miyuki NAGANO Akiko NAKAMA Akemi KAI Kazuyoshi YANO

Tokyo Metropolitan Institute of Public Health

Inhibitors in food samples may be responsible for the difficulty in detecting Norovirus (NV) by PCR. To detect NV more efficiently we employed additional bacterial treatment process before RNA extraction in the standard protocol. Ten strains of bacteria were examined with the modified method using oyster samples and Proteus vulgaris was found to be the most effective. By quantification of NV RNAs using real-time PCR recovery rates of NVs (GI/8 or GII/4) added to oyster suspensions using the modified method were compared with those recovered using the standard method. Recovery rates using the modified method with P. vulgaris were 45.9% for GI/8 and 21.3% for GII/4 while those using the standard method were 0.2% for GI/8 and GII/4.
Keywords: ノロウイルス , カキ , 妨害物質 , 細菌 , 回収率 , 検出法 ,
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