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結核集団感染事例における分子疫学的解析法としてのVariable Numbers of Tandem Repeats法の検討
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向川 純、三宅啓文、吉田 勲、保坂三継、矢野一好
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東京都健康安全研究センター
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Usefulness of Variable Numbers of Tandem Repeats Method as a Molecular Epidemiological Tool for Outbreak of Tuberculosis
Jun MUKAIGAWA Hirofumi MIYAKE Isao YOSHIDA
Mitsugu HOSAKA Kazuyoshi YANO
Tokyo Metropolitan Institute of Pubric Health
Restriction fragment length polymorphism (RFLP) method is now widely used as an epidemiological tool for outbreak of tuberculosis. But it needs at least one microgram of bacterial DNA for analysis and it takes over one month for bacterial culture to get enough DNA so it is hard to get quick results. We have been reporting arbitrarily primed PCR (AP-PCR) method variable numbers of tandem repeats (VNTR) method which are based on PCR method to get rapid results for typing of Mycobacterium tuberculosis. Here we report about VNTR method which is suitable for construction of genome database of Mycobacterium tuberculosis and is a technically easy method is compared to RFLP method about analyzing power. We examined discrimination power of each tandem repeat region using Mycobacterium tuberculosis strains isolated from outbreak cases of tuberculosis.
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| Keywords: |
結核集団感染 , RFLP法 , VNTR法 , |
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[PDF (395.2K)]
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第5巻 , 第1号 , (pp.15-23)
