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イヌの外耳におけるMalassezia 属菌の保有状況と分離株の分子生物学的解析
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千葉隆司１、畠山薫１、水谷浩志２、和宇慶朝昭１、高橋由美１、山田澄夫３、甲斐明美1、矢野一好1
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1東京都健康安全研究センター、2東京都動物愛護相談センター、3東京都健康安全研究センター (現)日本生活協同組合連合会
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Occurrence and Molecular Analysis of Malassezia spp. in the External Ear Canal of Dogs
Takashi CHIBA１ Kaoru HATAKEYAMA１ Hiroshi MIZUTANI２ Tomoaki WAUKE１ Yumi TAKAHASHI１ Sumio YAMADA３ Akemi KAI１and Kazuyoshi YANO１
１Tokyo Metropolitan Institute of Public Health
2Tokyo Metropolitan Animal Care and Consultation Center 3Tokyo Metropolitan Institute of Public Health at the original publication Japan Consumers' Co-operative Union at present
Malassezia yeasts are normal skin flora of human and animals. However hospitals have acquired infections caused by Malassezia spp. have been reported recently. The aim of this study was to evaluate the occurrence and DNA sequence diversity of Malassezia spp. in the external ear canal of dogs in the Tokyo Metropolitan Area.
As a result Malassezia spp. were isolated from 33 out of 52 (63. 5%) dogs. M. pachydermaitis was identified in 31 (59. 6%) specimens by rDNA sequence analysis. These 31 isolates were classified into 5 clusters by molecular phylogenetic analysis using ITS1 region. Three isolates from 3 out of 52 specimens were found to display a high rDNA region sequence similarity to that of M. japonica type strain.
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| Keywords: |
酵母 , Malassezia 属菌 , イヌ , 分子系統樹 , |
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[PDF (264.2K)]
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第5巻 , 第1号 , (pp.7-14)
