化学生物総合管理

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第4巻 , 第1号 , (pp.4-16)
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鳥インフルエンザ診断のための遺伝子検査システムの確立
貞升健志、新開敬行、長島真美、尾形和恵、吉田靖子、山田澄夫、矢野一好
東京都健康安全研究センター
Establishment of genetic diagnostic system for avian influenza in humans

Kenji SADAMASU Takayuki SHINKAI Mami NAGASHIMA Kazue OGATA Yasuko YOSHIDA Sumio YAMADA Kazuyoshi YANO

Poultry outbreaks due to highly pathogenic H5N1 avian influenza have been spreading from Southeast Asia to other countries of the world including Japan. More than 300 human cases of H5N1 avian influenza virus infection as a result of infected poultry-to-human transmission have been reported. As a recombinant or reassortant virus between avian influenza viruses (H5 H7 and H9) and human and/or swine virus capable of efficient human-to-human transmission could trigger the new influenza pandemic the H5N1 virus is being most cautiously monitored by the World Health Organization (WHO) as a possible new strain at present. If the patient who would have flu-like illness recently returned from the H5N1 prevalent area and contacted poultry rapid and accurate diagnosis of the patient is most important for health risk management. But appropriate diagnostic methods had not existed to diagnose H5N1 or human influenza virus. We developed “The Early Detection System for Avian Influenza” based on the real-time reverse transcriptase polymerase chain reaction (PCR) RT-nested PCR and Loop-mediated Isothermal Amplification (LAMP) and this system made it possible to detect and discriminate between avian and human influenza rapidly. Six cases suspected avian influenza have been examined by this system from 2005.
Keywords: 高病原性鳥インフルエンザウイルス , リアルタイムPCR , H5N1 , ネスティドPCR ,
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